In Vitro Antioxidant Assay of Different Phytoconstituents and Extraction Optimization of Phenolics from Cyperus rotundus L

Oxidative stress related disorders occur as a result of free radicals in the cellular organs. Antioxidants provide protection contrary to oxidative by counteracting through help different bioactive constituents present plants. The aim this study is find out quantitative estimation phytochemicals (phenolics, tannins, flavonoid, non-tannins, alkaloid and saponins), vitro antioxidant activity (2, 2-diphenyl-1-picryl-hydrazyl-hydrate, metal chelating, ferric reducing, superoxide dismutase total activity) parts Cyperus rotundus. Along with this, extraction polyphenolic compounds conventional non-conventional methods their optimization response surface methodology technique were also studied. As outcomes study, maximum amount phenolics content was found ethanolic fraction stem (37.88±0.94 mg gallic acid equivalent/g). root has shown highest flavonoid (0.24±0.03 QAE/g) non-tannin (21.55±2.43 equivalent/g) extract petroleum ether. alkaloids tannin flower i.e., 5.05±0.08 caffeine equivalent/g 76.28±0.62 tannic be better aqueous respectively. ethyl acetate reported greatest saponin (39.2±3.8 In case part half-maximal inhibitory concentration value 2, 2-diphenyl-1-picryl-hydrazyl-hydrate varies from 0.57-1.90 mg/ml 0.053-1.401 Antioxidant each plant ethanol water fluorescence recovery after photobleaching (0.403±0.009-0.886±0.02 mg/ml), chelation (0.010±0.0-0.306±0.001 mg/ml) capacity (0.030±0.0-0.317±0.002 mg/ml). Microwave assisted method suitable one for phenolic content. So, relationship between parameter determined evaluated plotting graphs. Polar extracts have show quantity secondary metabolites compared non-polar one. And among comparison methods, ultra sonication (nonconventional method) yield compound. shows that rotundus wide range showing properties can applied therapeutic applications.